I am using imageJ to count specific stained cells in an image. I have different pictures, at different wavelengths to be certain that I am counting the right type of cells.
For this I want stack the images so I can scroll through them while counting. I am using the point tool with “auto-measure” and “add to overlay” to count and to see which cells are already counted.
However, when I do this, it adds the overlay to one slice. I would like to add the overlay to all slices in my stack. I know that this is possible with for examples the pencil tool, by enabling “paint on overlay” but I can not use this to count my cells.
Does anyone have any suggestions how I can count, and see the overlay in all slices?