I am interested in setting up histocytometry for my project (and eventually CytoMAP). However, we are working on installing MatLab to the Imaris workstation so I currently do not have access to the “Channel Arithmetics” Xtension that the Gerner Lab uses for generating a spillover/compensation matrix. Does anyone know of a plugin/macros script for FIJI/ImageJ? (I don’t think Lumos is quite what I need).
Alternatively, is it acceptable to quantitate all channels in single fluorophore slides, export to csv, and perform compensation in FlowJo, then apply comp matrix to actual sample slides processed in the same way? Any thoughts @cstoltzfus?