I am new to cellprofiler. I have managed to use the speckle counting and translocation example pipelines to measure 1. No. of H2AX (Red channel) foci per nucleus, 2. Intensity of protein of interest -POI (GFP channel) in nuclei. 3. Ratio of POI (GFP channel) between nuclei and cytoplasm. But I am kind of lost after generating the spreadsheet.
I tried to use cellprofiler analyst to plot the per-object nuclei intensity against each well (scatter).
- How to analyse statistical significances? Is it required to learn R, Python etc., to make a proper plot?
- Is it possible to simply use the values from Spreadsheet generated in a graphpad or origin spreadsheet?
RWTH Aachen, Germany.