I’m using the plot file to measure the fluorescent intensity of my sample, and I found it’s inevitable to include the background since the plot profile requires line or rectangle selection. Like this in the image below, the black part is also included.
This is just part of the sample, so rotation cannot solve the problem to exclude the background. And the background included in the selected area is affecting the gray value here, the curve shape remains the same, but the Y value differs.
I’ve tried to use photoshop to make the background transparent, doesn’t work, background still counts in the gray value.
Could someone tell me if there’s a way to only measure the sample area in the plot profile? Or if I use the rectangle of the same size, then the results will be comparable?