Please help me with the CP pipelines

Hello everyone,

So I intended to use CP to quantify the amount of stained cells in the image of brainstem cross section. The tissue is double stained by immunohistochem. I tried a several example pipelines sample online but they did not work well under images with heavy background and result in low accuracy.

Does anyone have experience with this? Some tips, advices, and sample pipelines would be very helpful!

Thank you!

I hope the community can help! It’s hard to say without seeing an example image. Do you mind following the “If you create a new topic” suggestions?

Thank you for the reply! I would upload a picture once I got back to the lab for sure.