I’m new to macro writing and would love it if someone could help me here. I’m currently using a macro to analyse the size & shape of mitochondria in cells from B&W fluorescence images - this macro required thresholding of the image to a binary image. Is there a way to set up a macro to auto-threshold the image within the confines of 25% of maximal intensity and maximal intensity?
If it is not possible to do this in a macro - is there any other way of doing this?
For reference, I am using imageJ 1.41 (due to the age of the macro i’m using) on a Mac.
Any help or advice would be greatly appreciated!