Hello and happy spring there in Boston. We are at work on imaging placental tissue for an NIH RO1 application. We want to determine the degree to which the placenta being examined has vascular underperfusion. In order to do this, we must figure the vessel area per villus in which they are contained. Therefore we must count the number of villi and vessels per image, measure their area, relate the two areas and outline both villi and vessels. The PrimaryObjects are the vessels contained inside the villi, which are the SecondaryObjects. When I ColortoGray, the Primary(vessels) come out best in Blue image, while the Secondary(villi) come out best in Green image.
I need help on a number of issues:
- Perfecting the identification of primary and secondary objects. CP is getting stuck trying to do this.
- Relating the area of the vessels to the area of the villus they are contained in – to make sure they are properly associated and that I can perform correct calculation for percentages.
- Outlining so that we can view the outlines and check that CP is capturing the vessels and villi we want.
I’ve attached here a hand-marked scan of the image just for reference. I’ve circled and numbered the Secondary(villi) we want to count, and some Primary(vessels) inside each villi that we would include. I’ve also included the original image (and H+E stained slide), and the pipeline.
This is an important new aspect of our analysis, and we really appreciate your help.
Perinatal Pathology Research Assistant
Department of Pathology, Northwestern University Feinberg School of Medicine
Clinical Research Associate
Department of Pediatrics, Division of Neonatology
Ann and Robert H. Lurie Children’s Hospital
320 E. Superior St., Searle 4-490
Chicago, Illinois 60611
Placenta vesselvilli pipeline.cp (9.53 KB)
Marked up image showing villi and vessels.pdf (929 KB)