Pixelated neurons

imagej

#1

Hi, I was wondering why my neurons are lines and not wiggly lines??? Trying to skeletonise for sholl analysis.

Thanks.


#2

@catherine123

Perhaps you can post an original image, as well as an example of your skeletonization so we can see this ‘non-wiggly’ issue? Also - what is the exact workflow you are applying?

Then let’s see if we can better help you…

eta