I’m new to QuPath and impressed with what it can do, especially regarding cell detection.
However, what I have are slides with three components: fibrin, red blood cells (RBCs) and thrombocytes (platelets). RBCs and platelets don’t have nuclei and fibrin is an extracellular protein with an amorphous quality in H&E.
I have trained a pixel classifier to identify these three components for quantitative analys since none of them have nuclei. I used rectangular selections of various sizes, one at a time, in which I annotated the components. It does fairly well but not great.
- How do improve the classifier?
- In “Show annotation measurements”, is it possible to get the total area for each component, or do I have to copy the results to clipboard and paste it in excel?