I’m new to FIJI and I’m trying to measure the fluorescence of a phagosome membrane as it changes with time. I’d like to track the bacteria (particle) within the phagosome as it moves through multiple z stacks. The bacteria are fluorescent red. I’d then like to measure the fluorescence of a region of interest around the bacteria (the phagosome membrane). The phagosome membrane is fluorescent green. I was thinking about using the TrackMate-extras plugin offered by FIJI. Is this the best tool?