I have some cells that contain a label with bright perinuclear staining. The staining occurs as a band of speckles surrounding the nucleus (the nucleus itself is as dark as background). I have been trying to use CellProfiler modules to select the nucleus. I can identify the speckles as primary objects, and I was hoping to use the UnifyObjects module to unify all the speckles and highlight one cell’s nucleus. So far it doesn’t look so promising, since UnifyObjects does not add any pixels. In addition, from your help section:
The space between the speckles is usually completely black, so I’m having trouble even unifying the speckles. Do you have any other suggestions for rigging up something that might work?