I have the following setup: I take images of lung tissue in which I stained for two markers. I take like 50 images per tissue slice and I use CellProfiler to identify nuclei and positive cells, which works like a charm. Now I get a CSV file where the number of positive cells is given for each image. I add up the positive cells and nuclei for all images from the slice and then calculate the percentage of positive cells to nuclei. So far so good.
My question would be: Can I somehow do the adding-up process in CellProfiler? The slide number is parsed as Metadata and then all events from images with the same slide number are added up?
Is this possible?
Thanks a lot for the help!