Percentage comparison between images

I am trying to quantify cardimyocytes beating using individual frames from a video I took. I was hoping to find a way to compare each image with the original in a way that would give me a percentage difference but cannot find such a tool in CellProfiler - is there a way to do this?


Hi Abraham,

There’s not a super-straightforward way to do this, but one approach may be to use LoadImages to load in each frame and LoadSingleImage to load in the one frame that you want to compare against. Then you can use ImageMath to do the per-image subtraction, and then use one of the other modules to collapse this difference into a single value.