So, I am very new to this software and downloaded it only to try to accomplish this one task:
I am working with nephrin stained images (green). Each image needs to be analyzed by selecting the Region Of Interest (ROI) and analyzing the PERCENTAGE of GREEN that has been customarily threshold-ed by ME. The point of this is to analyze how much of the nephrin has leaked out of the glomerulus and into the tubules. Again, the only way of quantifying this is getting a percentage of green “stuff” that is within the ROI, which can only be known by acquiring a threshold of everything OUTSIDE of the ROI.
If you are interested in helping me with this, please reach out to me if I need to further clarify.