detecting peaks.txt (11.2 KB)
Hoping to get some help here, please.
I am running a reconstruction of 7 image stacks (of beads in agarose imaged on an OpenSpim setup) using the multiview reconstruction plugin in Fiji.
At the point of detecting interest points, after defining a suitable threshold for the difference of a Gaussian, I noticed peaks are only detected for the 7th stack, while a message of ‘no peaks detected’ is thrown up for other stacks. I was wondering if someone can help on how to move past this. I have attached a file with the error message.
The stacks are tiff files and are more than 4GB (except the 7th stack). Could this be the problem? I loaded them using the Image stacks (ImageJ opener)… when defining the XML file.
I had problems loading these images when using the SPIm bead based registration plugin. So I am guessing the challenge might be the known problem of imageJ with tiffs that are more than 4GB.