I have a little question regarding the path intensity Profile.
Is the principle is the same as the scan line in Fiji?
I attached a picture of what I have done. There is the ROI and the corresponding path profile.
So the “Point #” is all the pixels it’s measuring, the X, Y, Z, T the coordinates for that pixel, and then the channels.
So does this mean that the path profiler is measuring the average intensity for each column of pixels over the distance (like in “scan line” in Fiji)? Or is it giving the intensity of all the pixels inside the ROI?
Thank you in advance,