I am Jennifer Risso and I am working in the UVSQ UFR in the laboratory headed by Marie-Anne Rameix Welti. I am writing to you because we are a team in that we perform imaging analysis diarily and we want to improve our adapt our particle detection methodology on ICY software to our new experimental objective.
We usually identify fluorescent objects from a binary image by using a home made protocol in ICY by using the spot detector plugin. Basically we detect them by looking for a best threshold by aplying the Otsu Method. Our pathway looks basically like:
Best thershold > Thersholder > Wavelet spot detector > Filter ROI by size > ROI statistics
However, now we are interested in to detect specific objects (spot 2) that are located inside of our old detected spots (spot 1). It is important to say that each one of our target spots (1,2) are detected in the microscope in different channels. Therefore, we are interested in to know if it exists some strategy we can use to get it in the ICY software.
Basically, it could be interesting to detect and use our first ROIs (spot 1, chanel 1) as a region of interest to found (only inside this region) our spots number 2 located in channel 2.
It could be very interesting and useful to us your help. If you need more information about our objective or about the images we use as an input, please, do not hesitate to contact me.
Thank you in advance.
Have a nice day,