I created a macro to analyze particles in a cell and see the evolution (number + size) of the particles over time.
The Analyze Particles plugin worked great and summarize allowed me to immediately get the count of particles for each time points.
But, since the Analyze Particles works on Binary images, the intensity measurements don’t appear in the summary. I also tried BioVoxxel analyze particles which seems to work but then crash due an error in column 21 (?).
Is there any way to do it easily instead of looping through all the time points, counting the ROI in the ROI Manager and getting the mean intensity for each frames ?
Thanks for the help.