Paint skeleton in two different colours

AM-Skeleton-ganz-C1-2021-01-08-AM-KO#42-5uM Dye.lif - 15a-2.tif (SRM Q=100.0).tif (468.9 KB)

Hello everybody :slight_smile:

I would be really happy about some help! I have got that skeleton of a cell. In the end, I want to have a white background, a black cell surface membrane and all inner membranes should be red. How do I get that? I tried everything that I could think off, now I am looking forward to your suggestions. I struggle with the function “Overlay” but maybe the solution lies there? Is it somehow possible to paint an inner Roi in red, an outer roi in black and then merge back both?

Thanks a lot for your help.