I am working on a pipeline to measure the edge intensity of podocyte cells, and was hoping to use the OverlayOutlines module to verify my pipeline was accurately determining where the ‘edge’ of cells are. I’d made a post here about a year ago where someone showed me an example output from this module:
But when I use the module I am unable to get a similar output, and can only manage to get a single line (I know the object identification can use some work, I haven’t optimized it yet):
I haven’t seen any posts in this forum about this which makes me think I’m missing something obvious, but I’ve been struggling for a bit so was hoping someone could tell me how to get output more like the first image.
Thanks for any help!