I hope you can help me with the following problem:
I have two z-stack images of immunofluorescent stained brain slices. The slices are consecutive, meaning that the lowest part of the upper z-stack is theoretically followed by the first part of the lower z stack. My aim is to overlay both 3D images that the cells from the upper and lower stack match each other. I already tried this plugin (https://sites.google.com/site/qingzongtseng/template-matching-ij-plugin#downloads) but it does not work on windows 10.
Do you have any ideas?
Additional info: On my slices I have stained neurons and astrocytes that I could or want to use as landmarkers.