I am a new user of Cellprofiler.Thanks for providing such a wonderful software for image analysis.
My work is to analyze image clusters showing the fluorescence variation in time course. The fluorescence signal concentrates in the nuclei of the cells. In my analysis, i have to track the cells, do segmentation, quantify the integrated fluo intensity of each cell and finally draw different curves reflecting the variation of fluo of each individual cell. After i perform the identifyPrimaryObjects module, I have to execute the IdentifyObjectsManually to “delete some objects (cells)”, and the EditObjectsManually to “add some objects” because some cells are nearby the mitosis and adjacent.
So can i overlap these two groups pf objects together for the following fluo measurement? the current version seems to lack such a function.