Overexposure issue of mIHC images

Hello,

I got the mIHC images using inForm. I found that some biomarkers show higher staining intensity or even overexposed when those images were open in Qupath for analysis. This issue gave me some false positive cell populations. How to tackle this issue? Thanks in advance.

Best

Hello,

As per the forum guidelines, if you provide no example datasets nor screenshots of what you are talking about, I’m not sure anyone can help at this point.

Best
Oli

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I think that my expression was clear in my last post. The mIHC images are from clinical trial projects. I can not post here.
I am pretty sure this issue is not only I met. Any help will be appreciated.

I’m afraid the question isn’t very clear to me either, I am also unable to help without images or a lot more information.

Edit: this Tweet may be relevant: