Hello all: this has to be a simple process but I can’t figure it out. We have multichannel fluorescent images for which we would like to be able to visualize whether, for instance, a green cell is also red, by drawing a green outline around all the green cells and displaying it on the red channel. In this example, we would want the entire red image to remain visible, so I don’t think a green mask will work (although there are probably mask options I don’t know about). The way I used to do this in Canvas for Mac (which is now a shell of its former self) was by using the wand selection tool to select (in the above example) the green areas of interest, then converting the selection to a path, coloring the path green, and placing it on the red channel. I can’t figure out how to do this in ImageJ, although I suspect this is also possible, but any method that works would be fine.
Thank you for your help!