I’ve been using global two class Otsu’s in IdentifyPrimaryObjects to quantify the area of DAB-staining. However, for some reason the program ignores the upper bounds that I set for the intensity. When I manually check the intensity in test mode in the output window, I can see that the pixel intensities are above my thresholds, but they still count as positive.
Unfortunately, I user very high resolution pictures so I won’t be able to upload them here.
Thanks in advance.