I’m using FIJI to extract the profile of a line obtained on a photo of the interface water/sediment taken into a glass aquarium. After ticking Invert Y Coordinates, setting the origin, scaling the image, setting it to 8-bit, I isolated and selected the line adjusting the threshold.
The aim is to convert the profile to an exploitable graph with its coordinates using Analyze > Tools > Analyze Line Graph. The problem I get is the following:
[The black line is drawn on what should be the x axis according to the set origin (the x coordinates of the set origin is x = 0 pixel).]
Not only the line graph is inverted on the x axis but the threshold of sign change (that is valid on the image i.e. all y values below the black line are negative) concerns only x values, that should be always positive…
Could someone explain to me this behavior from Analyze Line Graph that I don’t understand? I get the same kind of problem if the Y coordinates are not inverted.