So, you are actually using QuPath ? So it does work under QuPath ?
I think for processing big 2d files, you won’t have anything better than QuPath, did you check the full video from Neubias academy ? There are really a lot of convenient tools, if you include the classifiers (Quantitative Pathology & BioImage Analysis: QuPath - [NEUBIASAcademy@Home] Webinar - YouTube).
Now your file can be opened with BigDataViewer, but not directly, because it is too big (>2G pixels) - meaning you can’t open it with
File > Open ... then with
Import Current Image.
But you can open it with
BigDataViewer-Playground, and for that, please check Big Data I: Visualisation, File Formats and Processing in FIJI - [NEUBIAS Academy @Home] Webinar - YouTube ( especially the BigDataViewer-Playground part ). That’s the procedure I explained in my first post. And it works to open your file in BigDataViewer playground. Do you manage to open it ? I tried with the file you gave me, and it works.
Now maybe it’s better if you explain what processign you want to achieve, because then you can get advice either in QuPath or in Fiji.