I have a newbie Qupath question. I can open a multichannel “component” image I acquired with a akoya polaris instrument:
(btw, Stardist with a model I trained worked great!)
In ImageJ I took that same image, split it into individual channels and saved those as tif. Later I made a “Stack” from some of the individual tif images then saved it as an 8-bit tif and opened that in Qupath. Why doesn’t Qupath recognize the stack is composed of different channels?
Thanks for the help! -John