Hello, I’m new to using Fiji/imageJ and am currently working on two tasks.
Measuring blood vessel width/diameter using a single OCT retina photo. Vessel analysis plugin I have found online does not work with a single 2d image. Does anyone know of a simple way to consistently measure the vessel diameter?
Creating a line between two points and then measuring the distance to 3 points perpendicular to that reference line. The first perpendicular line is at the midpoint, 2nd line is 100 micrometers temporal from the midpoint, and the 3rd line is 200 micrometers temporal from the midpoint. Below is an image with an example of how a previous study did it, but they do not go into detail about how it was accomplished. Any help or insight would be greatly appreciated!