Objects disappearing from top left corner

I’m not sure if this question has been asked before but I’m running into an odd problem with the ‘IdentifyPrimaryObjects’ module. I’m using ‘IdentifyPrimaryObjects’ to identify and count fluorescently labeled cells however periodically when the module actually segments and counts the cells, it ignores all cells in the upper left quadrant. I’ve attached several images to showcase this. I believe the problem centers around the ‘Typical diameter of objects’ input because I fiddled around with it and the problem went away when set from 10-40 pixel units but recurred when set to anything else. Any help with this would be appreciated.

Thanks!



Hi,

Hmm, this is definitely odd. I’ve not seen that behavior with any ‘Typical diameter …’ settings, and would suspect that there is some issue with a pre-processing step. Would you please post the pipeline that produced this? File > Export > Pipeline… and attach it here.

Thanks,
David