I have two questions. First, I am tracking the area and intensity of stained CHO cells over multiple frames. I have a pipeline that seems to be working well but today I noticed that for a set of images with two cells the object number would change over each frame. For example frame 1 would have cell 1, labeled 1, and cell 2, labeled 2, but then the next frame the labeles would switch. Is there a way to keep the object numbers consistent or control how CP numbers objects?
My second question is using lsm files. Currently, I open the lsm files in ImageJ and split them (3 Channels) and save the sequence of frames as multiple TIFF files. Is there a way to do this in CP? I tried using LoadImages in CP to open the lsm file but it only recognizes the first image and not the series of frames.
Thank you so much for your help.