Hi guys, I hope all goes well. I’d like to thank you for all your replies and help, and apologize for my lack of timely responses. I’ll aim to improve those numbers in time to come.
I have a case where I get what appears to be incorrect neightbor counts and wondered if I have things set wrong. I have two primary objects, Nuc and F480 marker, and I want to highlight the F480 objects touching the nuclei.
Here are the outlines:
Here are my settings:
And here is the result:
I would expect only some of the nuclei to have neighbors…? Where am I going wrong?
Thanks for your time.