Object Intensity Measurement

cellprofiler

#1

I’ve been using CP and when I use measure object intensity the output values are MUCH MUCH lower than the intensity values I get it I measure by hand in say, imageJ. This holds true for both integrated density and mean intensity. Is there something I’m missing here?


#2

Hi,

There was a similar discussion, (others may know better than me), but the reason is because CP scale the intensity range of an image to a range of 0-1, quite originally the values would range from 0-255 or 0-65535