I am currently performing an analysis based on the segmentation of cells using CellProfiler followed by classification with CPAnalyst. Since my images are quite big and so are my cells, I perform noise removal and primary object identification using a downsized (4x) version of my images with no significant loss of performance. My problem is I would like to perform the subsequent object measurements on the original images (not downsized) but since I did object identification on the downsized ones, my object coordinates/data… refers to these images and will not match the original ones.
My question is if there’s any way to overcome this problem, like “resizing” the objects. For me it’s really important to perform the measurements on the originals for classification purposes as well as the processing time reduction achieved by downsizing the images (it gets around 10x faster) since I’m doing high throughput microscopy.
I know that this may not have a straight solution with the current version of CellProfiler but thanks anyway for your help and for the great tool CellProfiler is.