I am doing object based co-localization analysis using Jacop plugin. I have some difficulty in interpreting the results.
for example I got this results:
Colocalization based on distance between centres of mass
Threshold for Image A=63; Image B=49
Particles size between 0 & 1228800
Image A: 1404 centre(s) colocalizing out of 3179
Image B: 1559 centre(s) colocalizing out of 5122
if you calculate the percent of coloclaiztion fro image A it is 44.1 % while for image B is 30.4%. why are not the same ? and what percents represent a high and good co-localization versus low and poor colocaliztion ?
Thanks in advance,