number of pixels with a grey level greater than 0

Hello everyone,
I need to know how many pixels (with a grey level < 0 ) we have in one image sequence but I don’t know how I should proceed.
Can anyone help me please ?

Thank you !

If your image is a gray-scale image, you can get the histogram. If it is a color image, you can either use the Color Histogram plugin or convert the image to gray-scale before using the histogram feature. If you are using Fiji, the Color Histogram plugin is already included with the base installation.


Hello. It works !
Thank you very much for your help !