I’m trying to calculate different parameters (see below) on AgNOR stained glass slides (at 100x mag). I tried to use the ImageJ plugin Intensity Ratio Nuclei Cytoplasm Tool" but as a kind forum user pointed out it is made for immunofluorescence and it will not provide any info on single cells.
Those are the parameters I will need to measure:
- nuclear area of single cells
- mean n of AgNORs per nucleus
- mean area of AgNORs per nucleus
- AgNOR area : nuclear area (ratio)
Can anybody provide any suggestions about which program (ImageJ, QuPath, others) should I use to do that? I am not very familiar with image analysis but eager to learn.
Thank you for your help.
23154032-2.tif (1.0 MB) That’s an example of what my images look like at 8-bit
Another example of an original picture