Nuclei problem

cellprofiler

#1

I use DAPI to label nuclei of neural tissue containing glia. The glial nuclei are slightly smaller on average and a lot brighter. I am having a difficult time training CellProfiler to distinguish the two. My interest is mainly in the neurons. Any ideas short of just labeling the cells with NeuN (a neuronal nuclei marker)?

I would be pleased to give out a sample image that I am working with, but I don’t have a website to post it to. I could E-mail it though.

Thanks.


#2

I would first play with MeasureObjectAreaShape and MeasureObjectIntensity modules, then you should be able to find parameters which distinguish your small and bright nuclei from the rest.

Mike


#3

This is still giving me grief. Does the IdentifyPrimaryAuto module have to identify nuclei for the program to work well. I read in the hlep that it just makes the cell identification better. I have tried primary objects as cells instead and that works better but not perfect.