Nuclei, cytoplasm and cell counting

cellprofiler

#1

Hi
I have been trying to analyse my image which contains 3 channel (red green blue) Red contains principle cell, and blue contains DAPI nuclei. Green channel is distributed in both in and outside of the blue.
I would like to count the number of cells, number of nuclei. I would also want to measure the intensity inside and outside of the nuclei. I found cell profiler might be the best option. Any suggestion would be highly appreciated.
Thanks in advance.

kind regards
Mamun
Queensland Brain Institute
The University of Queensland.


#2

Hi,

Allow me to point you to our tutorials and example pipelines to get you started (I think the “human cells” example is a great place to start for the question you described!). Once you’ve started working on a pipeline, if you continue to have questions please feel free to post your work so far and we’ll try to help you troubleshoot particular issues.

Best of luck!