Hello all. I have attached a pipeline where the goal is to quantify the number of nuclei within individual grid squares.
The attached image is of a mouse spinal cord with the nuclei stained. The basic pipeline I have written crops this image to a region I am interested in and then superimposes a grid on this image.
What I an having trouble with is identifying the primary objects correctly since they are of different intensities and also how to set up the export function so that I get the number of nuclei within each grid.
All help or input appreciated. Thanks in advance.