Sample image and/or code
The above shows an example of my data, where cells have been detected using stardist in qupath. As you can see it misses many of the cells that are positive for DAB. The DAB seems to be well seperated from the rest of the signal, but where there is strong DAB signal, the heamatoxylin is gone.
A quick question in between: Stardist doesn’t care about the staining vectors right? It needs rgb information for each pixel and that is send to the u-net.
Is there a way to improve the detection, maybe separate and remove the DAB signal prior to sending the RGB image to stardist. Or maybe “recolor” the DAB to have the color of haematoxylin?