1_MIP_TIFF_today.tiff (1.7 MB)
I have tiled .czi images that I want to stitch together. To do so, I save as .TIF files, and upload to Fiji.
However, once I open the file I realize it automatically opens them as RGB images. When I convert them to composite images, it only shows three channels (green, red, blue). I have four channels in my .CZI images, so it seems my far red and my red are being combined in Image J.
Is there a way to preserve all four channels? I read that you could have more than 3 channels on section 9 of the image J guide (ImageJ User Guide - IJ 1.46r | Color Images) but it’s too vague for me to understand how to make that happen.
Any help or tips would be greatly appreciated!