I have a tiny question
I try to find a strong protocol for normalisation of my multiple EM 3D stack. Also, I have a staining on these EM 3D stack.
I already tried to do the simple steps
1-normalisation of the contrast trough the stack
2-play with the display with the pic at the middle
And good to go
But this last step, I’m perplex with it because if it’s not consistent for all my stacks my stats at the end would be false. Since I’m doing stat of intensity correlate to the morphology at the end
I also know that each image in electron microscopy could be different for sure.
But I just want to be sure to have strong protocol to normalise my stack in the same way.
If you have any idea.