My experiment involves acquiring images at different time points and the analyse the fluorescence intensity from single cell. Note that the image frames taken at different time points are not the same. The expression of the protein is time-dependnet. I would like to plot a quantitative dot plot of time-dependent protein expression in single cell. In this case how do I normalise for fluorescence intensity from images taken at different time points? Is there any image analysis pipeline in CellProfiler? Any help would be much appreciated.