No segments detected - Nerve detection

Hi there! Thought the nerve detection feature was neat and tried to apply it to my research project. Nevertheless, I find it impossible to detect any nerve segment. I have followed every step the help tab mentions but cannot bring myself to detect the nervers crossing the junction. Ayn help?

Dear @Clara_Urena,
it’s hard to say what exactly went wrong without further details, but can you please open the ‘option’ menu within the ‘Nerve Detection’ tab and activate ‘Fluorescence image’ and disable ‘only junction crossing’ (just to be sure that’s not the problem) and let me know what happens after pressing ‘Detect’?

Cheers,
Manuel

I already did that. Still no nerves detected. Is there any more instruction to it than the handbook and the help tab in the nerve detection tool?