So i’ve uploaded an ICS file into Cell Profiler 2.1 and it recognizes all the metadata, however, once I start trying to split things up into various channels using image matching rules in NamesandTypes, it tells me “No Image Sets Available” when I update it. I’ve followed along exactly with this demonstration (cellprofiler.org/tutorials/L … movies.pdf), but to no avail. I’ve tried a bunch of permutations and nothing has helped. When I select only “MATCH->ALL->METADATA DOES HAVE C MATCHING #” for channels 0,1, and 2 separately it won’t work. Adding “MATCH->ALL->Image is Stack Frame” doesn’t help either UNLESS I select ANY. Additionally, only adding “MATCH->ALL->Image Is Stack Frame” will work, but I’m running into to problems later with that. For instance, when I try to identify primary objects using the object that refers to channel 2, it will start out calling the object that refers to channel 0…Now things will work if I only MATCH for one channel, however, I want to be able to access 2 channels. If someone could shed light on this situation I would appreciate it.