We had an issue where if the newer version of ICY (version 2 and higher) was used, the videos we wanted to analyse took extremely long and required more memory when passing through the intensity projection tool compared to the older version (version 1.8.0) which didn’t require as much memory and handled the videos (that were 4-8 seconds long) very quickly with the intensity projection tool. To give an example, we would allow a protocol to run for 14 hours and only 13 videos would have been completed. Could this be an issue with the newer ICY versions? Protocol is pasted below:
We also have a problem in that our protocol gives ROS numbers that differs from the ROS numbers obtained from manually reading the videos with ROS Statistics, even though the exact same parameters are being used. Example, for our drug controls we would identify 15 ROS centres but manually reading the video would show 0 centres. I saw this problem was already posted on the old ICY forum and had been solved however, the solution to the problem is somewhat unclear to me. Could you please elaborate on how to adjust our protocol so we could obtain the same results as manually reading the videos? Thank you in advance for your help in this matter.