Hello,
I would like to quantify the distance between microglia and blood vessels. Do you have any pipeline suggestion that I can use to quantify this?
Many Thanks,
Hello,
I would like to quantify the distance between microglia and blood vessels. Do you have any pipeline suggestion that I can use to quantify this?
Many Thanks,
I would recommend providing a sample image, or at least some idea of how you are generating your data.
Hi,
Thank you very much for your quick reply.
Please find attached the images (including nuclei)
The image is for IF (2D) and I would like to calculate the distance center to center.
Many Thanks,
Snap-07_b0c0x0-692y0-520.tiff (166.8 KB) Snap-07_b0c0x0-692y0-520.tiff_metadata.xml (335 Bytes) Snap-07_b0c1x0-692y0-520.tiff (190.4 KB) Snap-07_b0c1x0-692y0-520.tiff_metadata.xml (335 Bytes) Snap-07_b0c2x0-692y0-520.tiff (205.9 KB) Snap-07_b0c2x0-692y0-520.tiff_metadata.xml (335 Bytes)
Great! Hopefully someone conversant with CellProfiler will be along to help you I don’t actually use it, but wanted to make sure enough information was available for someone to help!
Hi @mtaga5510,
I don’t have already existing pipeline. But the workaround could be like this,
Regards,
Lakshmi
Fujifilm Wako Automation (Consultant)
www.wakoautomation.com
For CellProfiler training or optimised pipeline write to,
lakshmi.balasubramanian.contractor@fujifilm.com
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