Need help with automated cell counting!



I am new to ImageJ, and I need to be able to count the number of cells in a photomicrograph. Along with being able to know the total amount of cells, I also need to be able to identify the cells which are stained a darker blue color. I thought that maybe adjusting the threshold would be a good way to eliminate the lighter cells, but I haven’t been able to find a consistent threshold to use across multiple photos. Thanks!


Hello @Jacob_Kirby and welcome to the ImageJ forum!
It would be very helpful if

  1. You have a look a the previous posts on the same subject
  2. you post here an example image so we can play around with it and maybe offer you solutions.