I need to analyse different morphological properties of tomato seeds and create groups like:
seeds with a small area/Large area, oval shaped/round shaped (circularity) and light/darks seeds.
I already successfully analysed the area and cirularity with ImageJ, so I know how to select a segment of the full picture that I want to use, how to set the scale, covert the image to 8bit, adjust the threshold and analyse the particles. But I can’t seem to figure out how to get the color information of every individual seed so I can make 2 or 3 groups each within the different shades of brown present in the picture (ie. light/dark brown or light/medium/dark brown seeds).
The goal is to create 2 or 3 groups of color and sow these groups separately to see if there are any differences in seedling growth bewteen these groups. So any way to select the seeds by color is appreciated.